Metabolite Profile of arabica Coffee Cascara from Typica Cultivar in Bandung with Different Drying Processes

Authors

  • Zaki Fahreza Sururi Biology Study Program, Faculty of Mathematics dan Natural Sciences Education, Indonesia University of Education
  • Sariwulan Diana Biology Study Program, Faculty of Mathematics dan Natural Sciences Education, Universitas Pendidikan Indonesia
  • Kusdianti Kusdianti Biology Study Program, Faculty of Mathematics dan Natural Sciences Education, Universitas Pendidikan Indonesia

DOI:

https://doi.org/10.19184/jid.v26i2.52756

Keywords:

Caffeine, Direct sun, Gas Chromatography-Mass Spectrometry

Abstract

Arabica coffee bean (Coffea arabica L.) is Indonesia's leading commodity, with Bandung Regency as the largest producer in West Java. The exocarp and mesocarp of coffee fruits known as cascara have potential health benefits as a beverage. Cascara is usually dried by direct sunlight before consumption, but this process is prone to weather disturbances and contamination. The use of dehydrators is more controlled to maintain metabolite content, but there has been no research on cascara from coffee cultivars in Bandung Regency dried by this method. This study aims to obtain the metabolite profile of Typica arabica coffee cultivar cascara dried using direct sun and dehydrator. The cascara samples were taken from Gunung Puntang Coffee Plantation, Bandung Regency, and extracted using maceration method with 70% ethanol p.a. solvent. The metabolite content was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS), which specifically detects volatile metabolites. Non-volatile compounds were not within the scope of this study. The results showed that direct sun-dried cascara had 6 metabolites, while dehydrator-dried had 15 metabolites. Both samples were dominated by caffeine (sun-dried cascara: 46.61%, dehydrator cascara: 24.44%). Five metabolites were found in both samples, with 1 unique metabolite in solar cascara and 10 unique metabolites in dehydrator cascara. This study showed differences in metabolite content in Typica cultivar cascara with different drying methods.

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References

Aronson, J. & Ebeler, S. E. (2004). Effect of Polyphenol Compounds on the Headspace Volatility of Flavors. American Journal of Enology and Viticulture, 55(1), 13-21. https://doi.org/10.5344/ajev.2004.55.1.13

Arpi, N., Muzaifa, M., Sulaiman, M. I., Andini, R. & Kesuma, S. I. (2021). Chemical Characteristics of Cascara, Coffee Cherry Tea, Made of Various Coffee Pulp Treatments. IOP Conference Series: Earth and Environmental Science, 709(1), 012030. https://doi.org/10.1088/1755-1315/709/1/012030

Avato, P. & Tava, A. (2022). Rare Fatty Acids and Lipids in Plant Oilseeds: Occurrence and Bioactivity. Phytochemistry Reviews, 21(2), 401-428. https://doi.org/10.1007/s11101-021-09770-4

Badan Pusat Statistik (2023). Statistik Kopi Indonesia 2022. Jakarta: Badan Pusat Statistik.

Bobková, A., Poláková, K., Demianová, A., Belej, Ľ., Bobko, M., Jurčaga, L., Gálik, B., Novotná, I., Iriondo-DeHond, A. & Castillo, M.D. (2022). Comparative Analysis of Selected Chemical Parameters of

123 Coffea arabica, from Cascara to Silverskin. Foods, 11(8), 1082. https://doi.org/10.3390/foods11081082

Bondam, A. F., da Silveira, D. D., dos Santos, J. P. & Hoffmann, J. F. (2022). Phenolic Compounds from Coffee By-products: Extraction and Application in the Food and Pharmaceutical Industries. Trends in Food Science & Technology, 123, 172-186. https://doi.org/10.1016/j.tifs.2022.03.013

Csapó, J., Prokisch, J., Albert, C. & Sipos, P. (2019). Effect of UV Light on Food Quality and Safety. Acta Universitatis Sapientiae, Alimentaria, 12(1), 21-41. https://doi.org/10.2478/ausal-2019-0002

Dong, W., Hu, R., Chu, Z., Zhao, J. & Tan, L. (2017). Effect of Different Drying Techniques on Bioactive Components, Fatty Acid Composition, and Volatile Profile of Robusta Coffee Beans. Food Chemistry, 234, 121-130. https://doi.org/10.1016/j.foodchem.2017.04.156

Dudek, K., Pietryja, M. J., Kurkiewicz, S., Kurkiewicz, M., Błońska-Fajfrowska, B., Wilczyński, S. & Dzierżęga-Lęcznar, A. (2022). Influence of the Drying Method on the Volatile Component Profile of Hypericum perforatum Herb: A HS-SPME-GC/MS Study. Processes, 10(12), 2593. https://doi.org/10.3390/pr10122593

Fithriyyah, D., Wulandari, E. & Sendjaja, T. P. (2020). Potensi Komoditas Kopi dalam Perekonomian Daerah di Kecamatan Pangalengan Kabupaten Bandung. Mimbar Agribisnis: Jurnal Pemikiran Masyarakat Ilmiah Berwawasan Agribisnis, 6(2), 700-714. https://doi.org/10.25157/ma.v6i2.3408

Gemechu, F. G. (2020). Embracing Nutritional Qualities, Biological Activities, and Technological Properties of Coffee Byproducts in Functional Food Formulation. Trends in Food Science & Technology, 104, 235-261. https://doi.org/10.1016/j.tifs.2020.08.005

Heeger, A., Kosińska-Cagnazzo, A., Cantergiani, E. & Andlauer, W. (2017). Bioactives of Coffee Cherry Pulp and its Utilisation for Production of Cascara Beverage. Food Chemistry, 221, 969-975. https://doi.org/10.1016/j.foodchem.2016.11.067

Heo, J., Adhikari, K., Choi, K. S. & Lee, J. (2020). Analysis of Caffeine, Chlorogenic Acid, Trigonelline, and Volatile Compounds in Cold Brew Coffee Using High-Performance Liquid Chromatography and Solid-Phase Microextraction—Gas Chromatography-Mass Spectrometry. Foods, 9(12), 1746. https://doi.org/10.3390/foods9121746

Hu, D., Liu, X., Qin, Y., Yan, J., Li, R. & Yang, Q. (2023). The Impact of Different Drying Methods on the Physical Properties, Bioactive Components, Antioxidant Capacity, Volatile Components and Industrial Application of Coffee Peel. Food Chemistry: X, 19, 100807. https://doi.org/10.1016/j.fochx.2023.100807

Indrayani, N. M. K., Sunaryono, J. G. & Purwanti, E. W. (2022). Analisis Nilai Tambah Kulit Kopi Arabika (Coffea arabica) Sebagai Produk Olahan Teh Celup Cascara di Desa Taji Kecamatan Jabung Kabupaten Malang. Jurnal Pengolahan Pangan, 7(2), 67-74. https://doi.org/10.31970/pangan.v7i2.77

International Coffee Organization (2023). Coffee Report and Outlook December 2023. London: International Coffee Organization.

Iriondo-DeHond, A., Iriondo-DeHond, M. & del Castillo, M. D. (2020). Applications of Compounds from Coffee Processing By-Products. Biomolecules, 10(9), 1219. https://doi.org/10.3390/biom10091219

Jain, D. & Tiwari, G. N. (2003). Thermal Aspects of Open Sun Drying of Various Crops. Energy, 28(1), 37-54. https://doi.org/10.1016/S0360-5442(02)00084-1

Jiménez-Zamora, A., Pastoriza, S. & Rufián-Henares, J. A. (2015). Revalorization of Coffee By-products: Prebiotic, Antimicrobial and Antioxidant Properties. LWT - Food Science and Technology, 61(1), 12-18. https://doi.org/10.1016/j.lwt.2014.11.031

Kachroo, A. & Kachroo, P. (2009). Fatty Acid–Derived Signals in Plant Defense. Annual Review of Phytopathology, 47(1), 153-176. https://doi.org/10.1146/annurev-phyto-080508-081820

Kaido, B. & Takashino, N. (2023). Comparative Challenges, Cost, and Profitability of Cooperative versus Noncooperative Farmers: Case of Arabica Coffee in Indonesia. Food Research, 7(2), 297-306. https://doi.org/10.26656/fr.2017.7(2).510

Kaur, N., Chugh, V. & Gupta, A. K. (2014). Essential Fatty Acids as Functional Components of Foods - A Review. Journal of Food Science and Technology, 51(10), 2289-2303. https://doi.org/10.1007/s13197-012-0677-0

Komaria, N., Suratno, Prihatin, J. & Sudarti (2020). An Analysis of Innovation on the Utilization of Cascara by Coffee Farmers. Journal of Physics: Conference Series, 1563(1), 012015. https://doi.org/10.1088/1742-6596/1563/1/012015

Lestari, W., Hasballah, K., Listiawan, M. Y. & Sofia, S. (2023). Antioxidant and Phytometabolite Profiles of Ethanolic Extract from the Cascara Pulp of Coffea arabica Collected from Gayo Highland: A Study for Potential Anti-photoaging Agent. F1000Research, 12(1), 1-15. https://doi.org/10.12688/f1000research.120278.2

Lo, Y. & Hwang, S. (2024). Enhancing Natural Enemy Performance through Plant Secondary Metabolites: The Role of Caffeine for the Parasitoid Snellenius manilae. Journal of Applied Entomology, 148(3), 330–338. https://doi.org/10.1111/jen.13228

124

Lovestead, T. M. & Urness, K. N. (2019). Gas Chromatography/Mass Spectrometry. New York: ASM Handbook.

Mariamenatu, A. H. & Abdu, E. M. (2021). Overconsumption of Omega-6 Polyunsaturated Fatty Acids (PUFAs) versus Deficiency of Omega-3 PUFAs in Modern-Day Diets: The Disturbing Factor for Their “Balanced Antagonistic Metabolic Functions” in the Human Body. Journal of Lipids, 2021, 1–15. https://doi.org/10.1155/2021/8848161

Mróz, M., Parchem, K., Jóźwik, J., Domingues, M. R. & Kusznierewicz, B. (2024). The Impact of Different Drying Methods on the Metabolomic and Lipidomic Profiles of Arthrospira platensis. Molecules, 29(8), 1747. https://doi.org/10.3390/molecules29081747

Murlida, E., Noviasari, S., Nilda, C., Rohaya, S., Rahmi, F. & Muzaifa, M. (2021). Chemical Characteristics of Cascara Tea from Several Varieties of Coffee in Aceh Province. IOP Conference Series: Earth and Environmental Science, 667(1), 012078. https://doi.org/10.1088/1755-1315/667/1/012078

Pua, A., Choo, W. X. D., Goh, R. M. V., Liu, S. Q., Cornuz, M. E. K., Sun, J., Lassabliere, B. & Yu, B. (2021). A Systematic Study of Key Odourants, Non-volatile Compounds, and Antioxidant Capacity of Cascara (Dried Coffea arabica Pulp). LWT, 138, 110630. https://doi.org/10.1016/j.lwt.2020.110630

Rohloff, J. (2015). Analysis of Phenolic and Cyclic Compounds in Plants Using Derivatization Techniques in Combination with GC-MS-Based Metabolite Profiling. Molecules, 20(2), 3431–3462. https://doi.org/10.3390/molecules20023431

Tallima, H. & El Ridi, R. (2018). Arachidonic Acid: Physiological Roles and Potential Health Benefits – A Review. Journal of Advanced Research, 11, 33–41. https://doi.org/10.1016/j.jare.2017.11.004

Tenyang, N., Ponka, R., Tiencheu, B., Djikeng, F. T. & Womeni, H. M. (2020). Effect of Traditional Drying Methods on Proximate Composition, Fatty Acid Profile, and Oil Oxidation of Fish Species Consumed in the Far‐North of Cameroon. Global Challenges, 4(8), 2000007. https://doi.org/10.1002/gch2.202000007

Utami, N. F., Elya, B., Hayun & Kusmardi. (2022). Measurement of Quality Non-specific and Specific Parameters of 70% Ethanol Extract and Simplicia from Cascara Coffee Robusta (Coffea canephora L.) and Its Potency as Antioxidant. IOP Conference Series: Earth and Environmental Science, 1116(1), 012008. https://doi.org/10.1088/1755-1315/1116/1/012008

Wood, S., Sage, J. R., Shuman, T. & Anagnostaras, S. G. (2014). Psychostimulants and Cognition: A Continuum of Behavioral and Cognitive Activation. Pharmacological Reviews, 66(1), 193–221. https://doi.org/10.1124/pr.112.007054

Xiao, S., Li, H., Xu, M., Huang, K., Luo, Z. & Xiao, L. (2021). A High-throughput Method for Profiling Fatty Acids in Plant Seeds Based on One-step Acid-catalyzed Methylation Followed by Gas Chromatography-Mass Spectrometry. Biotechnology & Biotechnological Equipment, 35(1), 1076–1085. https://doi.org/10.1080/13102818.2021.1954552

Zioud, A., Hajji, W., Lobón, S., Joy, M., Bertolin, J. R., Smeti, S., Chabbouh, M., Bellagha, S. & Essid, I. (2023). Effects of Drying Methods on Chemical Composition, Lipid Oxidation, and Fatty Acid Profile of a Traditional Dried Meat Kaddid. Foods, 12(20), 3837. https://doi.org/10.3390/foods12203837

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Published

2025-07-29

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