Partial Analysis of Probiotic Character of Lactic Acid Bacteria from the Intestines of Broilers Supplemented with Fish Protein Hydrolysate (FPH)

Authors

  • Shafa Nashrullah Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jember, Indonesia
  • Eva Tyas Utami Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jember, Indonesia
  • Fina Yunita Purniasari Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jember, Indonesia
  • Dhanti Fatma Anggitasari Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jember, Indonesia
  • Esti Utarti Department of Biology, Faculty of Mathematics and Natural Sciences, University of Jember, Jember, Indonesia

DOI:

https://doi.org/10.19184/jid.v26i2.53701

Keywords:

Broiler, Fish protein hydrolysate (FPH), Lactid acid bacteria (LAB), Probiotics

Abstract

This study aims to analyze partial characteristics of probiotics lactic acid bacteria (LAB) from broiler intestines supplemented with fish protein hydrolysate (FPH) 2% (v/w) in feed. A total of 17 LAB isolates were successfully isolated and characterized morphologically and biochemically (catalase test). The isolate showed diverse morphological and biochemical properties, obtained as many as seven isolates that met the partial characteristics of probiotics, namely U10, U11, U13, U16, U31, U33, and U37 which were Gram-positive and no endospores and catalase formations were found. The tolerance test for acid pH and NaCl performed on the seven BAL isolates showed a diverse survival rate with U11 showing the highest tolerance at pH 2 (11.4%) and NaCl concentration up to 8% (32.4%). These findings highlight seven LAB isolates with partial probiotic properties, providing new insights into the development of probiotic candidates from broiler guts to improve gastrointestinal health and productivity.

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References

Adawiyah, S. R., Hafsan, H., Nur, F., & Mustami, M. K. (2015). Ketahanan bakteri asam laktat asal dangke terhadap garam empedu sebagai kandidat probiotik. In Prosiding Seminar Nasional Biologi (Vol. 1, No. 1).

Astuti, R. T., H. S. Yufidasari, H. Nursyam & Jessica, D. G. B. (2020). Isolasi Bakteri Asam Laktat dari Bekasam Ikan Patin dan Potensi Antimikrobanya terhadap Beberapa Bakteri Patogen. Journal of Fisheries and Marine Research, 5(3), 578-585.

Ayun, Q., Muthiah, S. N., & Sukmalara, D. (2023). Potensi Bakteri Asam Laktat (BAL) dari Jus Tempe sebagai Kandidat Probiotik. Jurnal Al-Azhar Indonesia Seri Sains dan Teknologi, 8(2), 171-177.

Belkis, M. (2023). Analisis probiotik asal usus ayam pedaging hasil perlakuan diversifikasi pakan menggunakan hidrolisat protein ikan. Skripsi. Jember: Program Studi Biologi Universitas Jember.

Betancur-Hurtado, et al. (2022). An in vivo pilot study on probiotic potential of lactic acid bacteria isolated from the gastrointestinal tract of Creole Hens (Gallus gallus domesticus) Native to Montería, Córdoba, Colombia in Broiler Chickens. Poultry. 1:157-168. DOI: 10.3390/poultry1030014.

Bogusławska-Tryk, M., Ziółkowska, E., Sławińska, A., Siwek, M., & Bogucka, J. (2021). Modulation of intestinal histology by probiotics, prebiotics and synbiotics delivered in ovo in distinct chicken genotypes. Animals, 11(11), 1-14. https://doi.org/10.3390/ani11113293

Chizhayeva, A., Amangeldi, A., Oleinikova, Y., Alybaeva, A., & Sadanov, A. (2022). Lactic Acid Bacteria as Probiotics in Sustainable Development of Aquaculture. Aquatic Living Resources, 35(10), 1-17.

Ditjen PKH. (2023). Statistik Peternakan dan Kesehatan Hewan 2023 Livestock and Animal Health Statistics 2023. Jakarta Selatan: Direktorat enderal Peternakan dan Kesehatan Hewan Kementrian Pertanian RI.

Gauthier, R. (2002). Intestinal health, the key to productivity (the case of organic acid). XXVII Convencion ANECA-WPDC. 30 April 2002. Jefo Nutrition.

Giyatno, D. C., & Retnaningrum, E. (2020). Isolasi dan karakterisasi bakteri asam laktat penghasil eksopolisakarida dari buah kersen (Muntingia calabura L.). Jurnal Sains Dasar, 9(2), 42-49.

Harumdewi, E., Suthama, N., Mangisah, I., Fakultas, D., Diponegoro, U., & Koesoemowardjojo, R. S. (2018). Pengaruh pemberian pakan protein mikropartikel dan probiotik terhadap kecernaan lemak dan perlemakan daging pada ayam broiler. Jurnal Sains Peternakan Indonesia, 13(3), 258-264. https://doi.org/10.31186/jspi.id.13.3.258-264

Jacobsen, C. N., V. R. Nielsen, A. E. Hayford, P. L. Moller, K. F. Michaelsen, A.P. Erregard, B. Sandstrom, M. Tvede, and M. Jakobsen. 1999. Screening of probiotic activities of forty seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in human. J. App. Environ. Microbiol.65: 4949-4956.

Khurajog, B., Disastra, Y., Lawwyne, L. D., Sirichokchatchawan, W., Niyomtham, W., Yindee, J., Hampson, D. J. & Prapasarakul, N. (2023). Selection and Evaluation of Lactic Acid Bacteria from Chicken Feces in Thailand as Potential Probiotics. PeerJ, 11, 1-22.

Khushboo, Karnwal, A., & Malik, T. (2023). Characterization and selection of probiotic lactic acid bacteria from different dietary sources for development of functional foods. Frontiers in Microbiology, 14, 1170725.

Lepecka, A., Szymanski, Rutkowsa, S., Iwanowska, K., & Krajewska, D. K. (2021). The Influence of Environmental Conditions on The Antagonistic Activity of Lactic Acid Bacteria Isolated from Fermented Meat Products. Foods, 10, 1-12.

Li, X., Li, W., Zhao, L., Li, Y., He, W., Ding, K., & Cao, P. (2024). Characterization and assessment of native lactic acid bacteria from broiler intestines for potential probiotic properties. Microorganisms, 12(4), 749.

Linares, D. M., Gomez, C., Renes, E., Fresno, J. M., Tornadijo, M. E., Ross, R. P., & Stanon C. (2017). Lactic Acid Bacteria and Bifidobacteria with Potential to Design Natural Biofunctional Health-Promoting Dairy Foods. Frontiers Microbiology, 8, 846.

Madigan, M. T., Bender, K. S., Buckley, D. H., Sattley, W. M., & Stahl, D. A. (2019). Brock Biology of Microorganisms : Fifteenth Edition. New York: Pearson.

Mansur, D. S., M. N. Hidayat dan Irmawaty. (2019). Ketahanan bakteri asam laktat asal saluran pencernaan broiler terhadap pH dan garam empedu. Jurnal Ilmu dan Industri Peternakan, 5(1), 27-37.

Mgomi, F. C., Yang, Y., Cheng, G., & Yang, Z. (2023). Lactic Acid Bacteria Biofilms and Their Antimicrobial Potential Against Pathogenic Microorganisms. Biofilm, 5, 1-12.

Mujnisa, A., Rotib, L. A., Djide, N., & Natsir, A. (2013). Ketahanan bakteri asam laktat hasil isolasi dari feses broiler terhadap kondisi saluran pencernaan broiler. JITP. 2(3):152-158.

Priadi, G., Setiyoningrum, F., Afiati, F., Irzaldi, R., & Lisdiyanti, P. (2020). Studi in vitro bakteri asam laktat kandidat probiotik dari makanan fermentasi indonesia. Jurnal Teknologi dan Industri Pangan, 31(1), 21–28. https://doi.org/10.6066/jtip.2020.31.1.21

Reuben, R. C., Roy, P. C., Sarkar, S. L., Alam, R. U., & Jahid, I. K. (2019). Isolation, Characterization, and Assessment of Lactic Acid Bacteria Toward Their Selection as Poultry Probiotics. BMC Microbiology, 19(253), 1-20.

Rizal, A. F. (2008). Hidrolisis Protein Ikan Lemuru (Sardinella sp.) menggunakan Ekstrak Kasar Protease dari Isi Perut Ikan Skipjack Tuna. Skripsi. Jember: Program Studi Kimia Universitas Jember.

Salamah, E., Nurhayati, T., & Widadi, I. R. (2012). Pembuatan dan karakterisasi hidrolisat protein dari ikan lele dumbo (Clarias gariepinus) menggunakan enzim papain. Jurnal Pengolahan Hasil Perikanan Indonesia,15(1), 9-16.

Salminen, S., Wright, A. V., & Ouwehand, A. (2004). Lactic acid bacteria microbiological and functional aspects. Marcell Dekker.

Samuel, O., Mavis, O., & Frederick, O. (2019). In Vitro Studies of the Probiotic Properties of Lactic Acid Bacteria Isolated from Akamu–A Nigerian Weaning Food. Immunol and Infect. Dis. 7(2): 13-20.

Singapurwa, N. M. A. S., Candra, I. P., & Semariyani, A. A. M. (2022). Profil protein ikan lemuru dengan pengeringan oven, pengering matahari dan sinar matahari berbasis sds page. Jurnal Teknologi Hasil Pertanian, 15(2), 83. https://doi.org/10.20961/jthp.v15i2.53612

Sirisopapong, M., Shimosato, T., Okrathok, S., & Khempaka, S. (2023). Assessment of lactic acid bacteria isolated from the chicken digestive tract for potential use as poultry probiotics. Animal bioscience. 36(8):1209.

Sjaifullah, A., Letitia, I. N., Adi W. I. N., & Oktavianawati, I. (2019). The effect of fish hydrolysate addition to the standard feed and its storage duration to the total content of nitrogen in the excreta of laying chicken. Jurnal Ilmu-Ilmu Peternakan, 29(3), 200–208. https://doi.org/10.21776/ub.jiip. 2019. 029.03.01

Tang, H., Huang, W., & Yao, Y. F. (2023). The Metabolites of Lactic Acid Bacteria: Classification, Biosynthesis and Modulation of Gut Microbiota. Microbial Cell, 10(3), 49-62.

Teul, S., Rahmawati & Ifadatin, S. (2023). Identification of Lactic Acid Bacteria from Fermented Kimchi Sawi Ansabi (Brassica juncea L.) using Phenotypic Similarities. Jurnal Biologi Tropis, 23(4), 50-60.

Tian, C., Wang, L., Liu, M., Liu J., Qiu, M., & Chen, Y. (2024). Isolation and Identification of Chicken-Derived Lactic Acid Bacteria: In Vitro Probiotic Properties and Antagonistic Effects Against Salmonella pullorum, Staphylococcus aureus, and Escherichia coli. Microorganism, 12, 795.

Wahyuni, S., & Putra, R. P. (2023). Kajian Minimum Inhibitor Concentration (MIC) dan Minimum Bactericidal Concentration (MBC) Ekstrak Kulit Terong Ungu (Solanum melongena L) Sebagai Pengembangan Antibakteri Herbal. Jurnal Pendidikan Teknologi Pertanian, 9(2), 249-262.

Wang, L., Lin, Z., Ali, M., Zhu, X., Zhang, Y., Li, S., Li, K., Kebzhai, F., & Li, J. (2023). Effect of Lactic Acid Bacteria Isolated from Tibetan Chickens on The Growth Performance and Gut Microbiota of Broiler. Frontiers in Microbiology, 14, 1171074.

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Published

2025-07-29

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