SCREENING AND IDENTIFICATION ALKALY-CELLULOLYTIC MOLDS FROM RICE STRAW ON COASTAL-FIELD OF WATU ULO JEMBER

Authors

  • Esti Utarti
  • Su’udah Hasanah
  • Siswanto Siswanto

DOI:

https://doi.org/10.19184/jid.v13i1.634

Keywords:

Screening, alkaly-cellulolytic molds, rice straw, coastal-field, A. terreus

Abstract

About 28 molds were obtained from rice straw on coastal-field of Watu Ulo Jember, were screened for their cellulolytic activities at pH alkaline. In semiquantitatively screening on CMC and Avicel plate at pH 8 showed that 27 isolates have cellulolytic activities. Based on clearing zone in CMC plate, isolates 7, 9, 14, 19 and 24 have higher cellulase (CMC-ase) activities index at pH alkaline. Further, in quantitatively examination using rice straw in Basic Salt Mandel’s modification medium showed isolate 19 (0,60 U/ml) that identified as Aspergillus terreus have higher FP-ase activity than isolate 7  (0,56 U/ml), 9  (0,55 U/ml), 14 (0,53 U/ml) and 24 (0,56 U/ml).

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General